BIOCHEMICAL AND CYTOLOGICAL EXAMINATION ON THE INITIATION OF RIBOSOMAL RNA SYNTHESIS DURING GASTRULATION OF XENOPUS LAEVIS
- 31 December 1975
- journal article
- research article
- Published by Wiley in Development, Growth & Differentiation
- Vol. 18 (4) , 329-338
- https://doi.org/10.1111/j.1440-169x.1976.00329.x
Abstract
Embryos of X. laevis at stage 6 were labeled with 14CO2 for 4 h and then allowed to develop under nonradioactive conditions until they reached stages 9, 10, 11 or 12. RNA was extracted and electrophoresed on a polyacrylamide-agarose gel. From the pattern of newly synthesized RNA, the incorporation into 18S and 28S RNA was measured. The specific radioactivity of nucleoside triphosphates in the acid-soluble fraction was determined. The absolute amounts of the RNA synthesized were calculated. The synthesis of rRNa began, or is at least markedly activated, around stage 10. Cytological examination showed that cells with nucleolated nuclei appeared at stages 9-10 and increased thereafter. The initiation of 18S and 28S RNA synthesis took place around stage 10.This publication has 22 references indexed in Scilit:
- Changes in RNA in relation to growth of the fibroblast. I. Amounts of mRNA, rRNA, and tRNA in resting and growing cellsCell, 1974
- Ribosomal RNA synthesis in pre- and postgastrula embryos of xenopus laevisCell Differentiation, 1972
- Initiation of the synthesis of ribosomal ribonucleic acid precursor in different regions of frog (Rana pipiens) gastrulaeBiochemical Journal, 1972
- The synthesis of 5-S RNA and its regulation during early embryogenesis of Xenopus laevisBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971
- Ribosomal RNA Synthesis and the Multiple, Atypical Nucleoli in Cleaving EmbryosScience, 1971
- Polyacrylamide gel electrophoresis of nucleic acids synthesised during the early development of Xenopus laevis DaudinBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1970
- Synthesis and accumulation of DNA-like RNA during embryogenesis of Xenopus laevisJournal of Molecular Biology, 1966
- Deoxyribonucleic acid in amphibian eggsJournal of Molecular Biology, 1965
- A study of the developmental cytology of a mutation affecting nucleoli in Xenopus embryosDevelopmental Biology, 1964
- RNA synthesis during the development of Xenopus laevis, the South African clawed toadJournal of Molecular Biology, 1964