The fruitfly Drosophila melanogaster contains a novel charged adipokinetic-hormone-family peptide
- 15 July 1990
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 269 (2) , 315-320
- https://doi.org/10.1042/bj2690315
Abstract
A member of the RPCH/AKH (red-pigment-concentrating hormone/adipokinetic hormone) family of arthropod neuropeptides was identified in the fruitfly Drosophila melanogaster, and its structure was determined by automated Edman degradation and m.s. using fast-atom-bombardment ionization and a tandem hybrid instrument capable of high sensitivity. The sequence of this peptide, which we call ‘DAKH’, is pGlu-Leu-Thr-Phe-Ser-Pro-Asp-Trp-NH2 (where pGlu is pyroglutamic acid and Trp-NH2 is tryptophan carboxyamide). H.p.l.c. analyses of extracts of the three body segments revealed that more than 80% of the peptide is contained in the thorax. Although DAKH is typical of family members in its general structure and distribution in the animal, it is unique in containing a residue which is charged under physiological conditions. The evolutionary significance of this change is considered.This publication has 28 references indexed in Scilit:
- Identification and characterization of adipokinetic hormone (locusta migratoria)-like immunoreactivity in the human cerebrospinal fluidBiochemical and Biophysical Research Communications, 1989
- Isolation and primary structure of a neuropeptide hormone from Heliothis zea with hypertrehalosemic and adipokinetic activitiesBiochemical and Biophysical Research Communications, 1988
- Sequence analyses of two neuropeptides of the AKH/RPCH-family from the lubber grasshopper, Romalea micropteraPeptides, 1988
- Primary sequence analysis by fast atom bombardment mass spectrometry of a peptide with adipokinetic activity from the corpora cardiaca of the cricket GryllusbimaculatusBiochemical and Biophysical Research Communications, 1987
- Insect hypertrehalosemic hormone: Isolation and primary structure from Blaberusdiscoidalis cockroachesBiochemical and Biophysical Research Communications, 1986
- Isolation and primary structure of a peptide from the corpora cardiaca of Heliothis zea with adipokinetic activityBiochemical and Biophysical Research Communications, 1986
- Sequence analyses of adipokinetic hormones II from corpora cardiaca of Schistocerca nitans, Schistocerca gregaria, and Locusta migratoria by fast atom bombardment mass spectrometryBiochemical and Biophysical Research Communications, 1986
- Amino acid sequence of Manduca sexta adipokinetic hormone elucidated by Combined Fast Atom Bombardment (FAB)/Tandem Mass SpectrometryBiochemical and Biophysical Research Communications, 1985
- Primary Structures of Locust Adipokinetic Hormones IIBiological Chemistry Hoppe-Seyler, 1985
- Structures of two cockroach neuropeptides assigned by fast atom bombardment mass spectrometryBiochemical and Biophysical Research Communications, 1984