HISTOCHEMICAL DEMONSTRATION OF SPECIFIC PHOSPHATASES OF THE LIVER PRESERVED IN HYPERTONIC SUCROSE SOLUTION

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Abstract
The observation of Holt that the storage of formalin fixed tissues in sucrose-gum acacia solution preserves acid phosphatase activity was confirmed. The same was found for 5-nucleotidase. Adenosinetriphosphatase activity is present after storage both in formalin and in sucrose-gum acacia solution. However, the staining for acid phosphatase activity after preservation in sucrose and for adenosinetriphosphatase activity after preservation in formalin and in sucrose solution revealed artefacts decreasing the usefulness of this procedure for routine application.

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