Abstract
Previous studies have demonstrated the presence of myoid cells in monolayers derived from cultured testicular fragments. In order to determine if monolayers of myoid cells can also be produced by other types of tissue cultures, monolayers derived from fragments of mouse adrenal capsules, cultured in McCoys modified 5A medium, were examined for the presence of myoid cells. The cells of the resultant monolayers contained numerous free ribosomes and abundant profiles of granular endoplasmic reticulum, as well as 40–60 Å thick filaments and associated dense bodies. In these respects they are identical to myoid cells found in monolayers in testis cultures. Contrary to earlier reports indicating that the adrenal capsule is composed of fibroblasts, this study demonstrates that myoid cells are a major component of the capsule of the rat and mouse adrenal gland.