Improved base calling for the Illumina Genome Analyzer using machine learning strategies
Open Access
- 14 August 2009
- journal article
- research article
- Published by Springer Nature in Genome Biology
- Vol. 10 (8) , R83
- https://doi.org/10.1186/gb-2009-10-8-r83
Abstract
The Illumina Genome Analyzer generates millions of short sequencing reads. We present Ibis (Improved base identification system), an accurate, fast and easy-to-use base caller that significantly reduces the error rate and increases the output of usable reads. Ibis is faster and more robust with respect to chemistry and technology than other publicly available packages. Ibis is freely available under the GPL from http://bioinf.eva.mpg.de/Ibis/.This publication has 7 references indexed in Scilit:
- Accurate whole human genome sequencing using reversible terminator chemistryNature, 2008
- Probabilistic base calling of Solexa sequencing dataBMC Bioinformatics, 2008
- Alta-Cyclic: a self-optimizing base caller for next-generation sequencingNature Methods, 2008
- Shotgun bisulphite sequencing of the Arabidopsis genome reveals DNA methylation patterningNature, 2008
- SOAP: short oligonucleotide alignment programBioinformatics, 2008
- 10.1162/15324430260185628Applied Physics Letters, 2000
- Base-Calling of Automated Sequencer Traces Using Phred. II. Error ProbabilitiesGenome Research, 1998