Unbiased Statistical Analysis for Multi-Stage Proteomic Search Strategies
- 30 November 2009
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Proteome Research
- Vol. 9 (2) , 700-707
- https://doi.org/10.1021/pr900256v
Abstract
“Multi-stage” search strategies have become widely accepted for peptide identification and are implemented in a number of available software packages. We describe limitations of these strategies for validation and decoy-based statistical analyses and demonstrate these limitations using a set of control sample spectra. We propose a solution that corrects the statistical deficiencies and describe its implementation using the open-source software X!Tandem.Keywords
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