Xylanibacterium ulmi gen. nov., sp. nov., a novel xylanolytic member of the family Promicromonosporaceae

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Abstract
A bacterial strain designated XIL08Twas isolated from an elm tree affected by Dutch elm disease. Strain XIL08Tis Gram-positive, aerobic, rod-shaped and non-motile. The complete 16S rDNA sequence of this micro-organism was obtained and phylogenetic analysis based on the neighbour-joining method indicated that the closest related organism belongs to the genusXylanimonasof the familyPromicromonosporaceae, suborderMicrococcineae. Cell-wall analyses revealed the presence of type A4α,l-lys–l-ala–d-Glu peptidoglycan. The cell-wall sugars found were rhamnose in large amounts, fucose, mannose and galactose and traces of arabinose and glucose. HPLC analysis of menaquinones revealed two peaks, the main peak corresponding to MK-9(H4) and the smaller one to MK-8(H4). The major fatty acid found was anteiso-C15 : 0. Mycolic acids were absent. The polar lipids detected were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. The G+C content of the DNA was 72 mol%. Isolate XIL08Thydrolysed xylan but not cellulose. Growth was observed with many carbohydrates including acetate and xylan as the only carbon source. Catalase activity was not detected. The data from this polyphasic study suggest that this bacterium belongs to a novel genus of the familyPromicromonosporaceae. It is proposed that isolate XIL08T(=LMG 21721T=CECT 5731T) be classified in a new genus,Xylanibacteriumgen. nov., as the type strain ofXylanibacterium ulmisp. nov.

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