Studies on the Neutralization of Herpes Simplex Virus

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Abstract
Herpes virus was reacted with an early rabbit antiserum containing predominantly complement‐requiring neutralizing (CRN) antibody to produce CRN‐antibody‐sensitized virus (SV), and the action of complement (C') upon SV was studied. Reduction of infectivity due to C' was about equal with undiluted and 1000‐fold diluted SV. Even higher dilutions which contained about 10 to 100 infectious units per 0.05 ml were also completely inactivated by C'. Kinetic experiments revealed that the velocity of titer reduction in the presence of C' of 100‐fold diluted SV was not slower than that of undiluted SV. When SV was first treated with C' and then diluted 100‐fold, the surviving virus showed but a slightly reduced efficiency of filtration through the 0.45 μ Millipore membrane as compared with SV first diluted 1: 100 and then treated with C'. The titer reduction of SV–C'1 complexes in the presence of C'4 followed a one‐hit curve. These results indicated that the reduction of infectivity of SV due to C' was not a result of immunoaggregation of infectious SV. Alternative possible mechanisms of the action of C' are discussed.