ELECTRON MICROSCOPE STUDIES OF BACTERIOPHAGE ACTIVE AGAINST STREPTOCOCCUS LACTIS

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Abstract
Electron micrographs of bacteriophage active against Streptococcus lactis have been made from material mounted on collodian membranes and shadowed with gold at an angle of 75[degree]. The phage particles are sperm-shaped, 220 m[mu] long, have a head diam. of 70 m[mu], and a tail that is 30 m[mu]. wide and 150 mu long. Nine strains of phage observed are so nearly alike in shape and size they cannot be differentiated on that basis. The 16 micrographs show what are believed to be different steps in the multiplication of the phage in a bacterial culture. The bacteria and phage were incubated in a 5% whey soln. at 30[degree]C. The S. lactis cells in contact with homologous phage appear to elongate prior to bursting.

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