Tumor necrosis factor bifunctionally regulates matrix metalloproteinases and tissue inhibitor of metalloproteinases (TIMP) production by human fibroblasts
Open Access
- 20 August 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 269 (1) , 93-95
- https://doi.org/10.1016/0014-5793(90)81127-a
Abstract
The production of tissue inhibitor of metalloproteinases (TIMP) in human uterine cervical fibroblasts was increased by human recombinant tumor necrosis factor α (hrTNF) at a low concentration (0.005 ) but the elevated synthesis was suppressed in a dose‐dependent manner at higher concentrations (up to 50 ). In contrast, the production of collagenase (EC 3.4.24.7) and stromelysin was stimulated at all the corresponding concentrations. In contrast, human recombinant interleukin‐1α (hrIL‐1, 10 ) coordinately induced these enzymes and TIMP production. The reduction of the elevated TIMP production by TNF was not due to the inhibition of TIMP secretion. These results suggest that TNF modulates the extracellular matrix degradation in human fibroblasts bifunctionally by the suppression of TIMP production in addition to the acceleration of matrix metalloproteinases production. Furthermore, the fact that TNF and IL‐1 differently controlled the production of TIMP suggests that the signal pathway of TNF for TIMP production is different from that of IL‐1.Keywords
This publication has 15 references indexed in Scilit:
- Evidence for metalloproteinase and metalloproteinase inhibitor imbalance in human osteoarthritic cartilage.Journal of Clinical Investigation, 1989
- Calmodulin regulates the interleukin 1-induced procollagenase production in human uterine cervical fibroblastsBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1989
- Human endothelial cell response to lipopolysaccharide, interleukin-1, and tumor necrosis factor is regulated by protein synthesisCellular Immunology, 1989
- Evidence that human rheumatoid synovial matrix metalloproteinase 3 is an endogenous activator of procollagenaseArchives of Biochemistry and Biophysics, 1988
- Detection of tumor necrosis factor α but not tumor necrosis factor β in rheumatoid arthritis synovial fluid and serumArthritis & Rheumatism, 1988
- Tumor necrosis factor stimulates gelatinase and collagenase production by granulation tissue in cultureBiochemical and Biophysical Research Communications, 1987
- Effects of dehydroepiandrosterone sulphate on the production of collagenase and gelatinolytic metalloproteinase by rabbit uterine cervical cells in primary cultures.Journal of Pharmacobio-Dynamics, 1986
- Cachectin/tumor necrosis factor stimulates collagenase and prostaglandin E2 production by human synovial cells and dermal fibroblasts.The Journal of Experimental Medicine, 1985
- Tumor Necrosis Factor (TNF)Science, 1985
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970