Glucosylation of chimeric proteins in the cell wall of Saccharomyces cerevisiae

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Abstract
Extension of a reporter protein with the carboxyterminal thirty amino acids of the cell wall mannoprotein α-agglutinin of Saccharomyces cerevisiae resulted in incorporation of the chimeric protein in the cell wall. By Western analysis it was shown that the incorporated protein contained β-1,6-glucan similar to endogenous cell wall proteins, whereas excreted reporter protein was not glucosylated. This suggests that β-1,6-glucan is involved in anchoring mannoproteins in the cell wall.