Detection of monoclonal immunoglobulins by immunoelectrophoresis: a possible source of error.

Publisher Website
Google Scholar
Abstract
The immunoelectrophoresis technique (IEP) is widely employed in the qualitative analysis of serum immunoglobulins (Ig). The most commonly used support media are agarose or agar gels, but Ig mobility is different in these 2 media. The presence of a small amount of a cathodal monoclonal IgG may not be detected on IEP in agar if it is masked by larger amounts of polyclonal Ig of the same class. In these circumstances the use of agarose imparts to the monoclonal protein a different mobility from that of the bulk of the serum IgG and allows its positive identification.