An Improved Purification Procedure for Sulfite Oxidase from Bovine Liver
- 1 March 1991
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 21 (1) , 53-61
- https://doi.org/10.1080/10826069108021515
Abstract
Sulfite oxidase (Sulfite:O2 oxidoreductase, EC 1.8.3.1) has been purified 2,440-fold from bovine liver. The procedure developed was used to isolate the enzyme from 1,000 g of liver and permitted the rapid isolation of enzyme with a very high specific activity (40,405 mU/mg). The enzyme preparations obtained have been characterized by electrophoretic and spectrophotometric analysis and the molecular mass and the Stokes radius of the enzyme have been determined.Keywords
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