Isolation and characterization of two distinct lectins with antiproliferative activity from the cultured mycelium of the edible mushroom Tricholoma mongolicum
- 1 December 1995
- journal article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 46 (6) , 508-513
- https://doi.org/10.1111/j.1399-3011.1995.tb01606.x
Abstract
Two lectins, TML-1 and TML-2, were isolated from Tricholoma mongolicum by ion-exchange chromatography and gel filtration. They are dimers with molecular weight near 37000. The hemagglutinating activities of TML-1 and TML-2 are sensitive to lactose inhibition and are stable between 10 and 80 degrees C. They exhibit antiproliferative activities against mouse monocyte-macrophage PU5-1.8 cells and mouse mastocytoma P815 cells in vitro. The two lectins differ in the content of proline and tyrosine residues. Both are non-glycoproteins and have hydroxyproline residues.Keywords
This publication has 19 references indexed in Scilit:
- Agglutinins (lectins) from some british higher fungiMycological Research, 1994
- A sialic acid‐binding lectin from the mushroom Hericium erinaceumFEBS Letters, 1994
- Isolation and Characterization of a Novel Ribosome-Inactivating Protein, β-Kirilowin, from the Seeds of Trichosanthes kirilowiiBiochemical and Biophysical Research Communications, 1994
- Plant Lectins as Inhibitors of Tumour Growth and Modulators of Host Immune ResponseChemotherapy, 1994
- Isolation and characterization of a lectin from Grifola frondosa fruiting bodiesBiochimica et Biophysica Acta (BBA) - General Subjects, 1990
- Purification and properties of a β‐galactosyl‐specific lectin from the fruiting bodies of Ischnoderma resinosusFEBS Letters, 1988
- Mistletoe Lectins and Their A and B ChainsOncology, 1986
- Studies of lectins XXXVI. Properties of some lectins prepared by affinity chromatography on O-glycosyl polyacrylamide gels☆Biochimica et Biophysica Acta (BBA) - General Subjects, 1978
- Lectins: Cell-Agglutinating and Sugar-Specific ProteinsScience, 1972
- Abrin and Ricin: New Anti-tumour SubstancesNature, 1970