Vasoactive intestinal polypeptide: a putative transmitter in the canine gastric muscularis mucosa.

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Abstract
The nature of the inhibitory transmitter in the canine gastric muscularis mucosae was studied in vitro using superfusion techniques. The inhibitory effect of nerve stimulation (10 V, 200 .mu.s, 10 Hz) was not altered by adrenergic, cholinergic or serotonergic antagonists. ATP had no effect on spontaneous mechanical activity. Nucleotide pyrophosphatase and apamin had no effect on the response to nerve stimulation. .alpha.-Chymotrypsin abolished the inhibitory effect of nerve stimulation. Radioimmunoassay of the muscle indicated the presence of gastrin/cholecystokinin-substance P- and vasoactive intestinal polypeptide (VIP)-like immunoreactivity. Of the 3 peptides present, only VIP produced a concentration-dependent relaxation. A substance with VIP-like immunoreactivity was released during nerve-induced relaxation of the muscle, and its release was blocked by tetrodotoxin and Ca-depleted solution. The inhibitory effect of nerve stimulation was abolished by VIP antiserum. VIP or a closely related peptide is apparently an inhibitory neurotransmitter in the canine gastric muscularis mucosae.

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