Rat Granulosa Cells Express Transforming Growth Factor-β Type 2 messenger Ribonucleic Acid which is Regulatable by Follicle-Stimulating HormoneIn Vitro
- 1 March 1990
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 126 (3) , 1777-1779
- https://doi.org/10.1210/endo-126-3-1777
Abstract
Freshly harvested granulosa cells (GC) from diethylstilbestrol (DES)-treated rats were examined for the presence of transforming growth factor-.beta.1 (TGF-.beta.1) and TGF-.beta.2 mRNA by Northern analysis. TGF-.beta.1 mRNA was not detectable, but hybridization of total RNA with a radiolabeled TGF-.beta.2 cDNA probe revealed two mRNA species (5.1 and 3.6 kb) indicative of TGF-.beta.2 mRNA. In response to FSH (50 ng/ml), relative TGF-.beta.2 concentrations in cultured GC were 54% of control levels. Furthermore, the conditioned culture medium from FSH-treated GC contained significantly lower (p < 0.01) TGF-.beta.-like activity relative to controls. These results demonstrate that rat GC express TGF-.beta.2 mRNA which is regulatable by FSH in vitro.Keywords
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