Presence and subcellular localization of two distinct mitogenic fractions in the cells of Nocardia rubra and Nocardia opaca: preparation of soluble mitogenic peptidoglycan fractions
- 1 April 1976
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 13 (4) , 1084-90
- https://doi.org/10.1128/iai.13.4.1084-1090.1976
Abstract
Fractionation of cells of Nocardia rubra and Nocardia opaca led to the separation of the cell wall and a "cytoplasmic" fraction. Both fractions were mitogenic for the splenocytes of AKR and nude mice and of rabbits. The peptidoglycan was the active part of the cell wall fraction. The products solubilized by the action of Streptomyces albus G peptidases on the peptidoglycan of N. rubra were mitogenic, but the products solubilized by lysoyme were not. Tentative structures are proposed for these fractions. The most active part of the cytoplasmic fractions could be sedimented by centrifugation and seemed to be related to the cytoplasmic membrane.Keywords
This publication has 15 references indexed in Scilit:
- Cell walls of mycobacteria and related organisms; Chemistry and immunostimulant propertiesMolecular and Cellular Biochemistry, 1975
- Occurrence of D-alanyl-(D)-meso-diaminopimelic acid and meso-diaminopimelyl-meso-diaminopimelic acid interpeptide linkages in the peptidoglycan of MycobacteriaBiochemistry, 1974
- Blastic Transformation of Mouse Spleen Lymphocytes by a Water-Soluble Mitogen Extracted from NocardiaProceedings of the National Academy of Sciences, 1974
- Adjuvant activity of monomeric bacterial cell wall peptidoglycansBiochemical and Biophysical Research Communications, 1974
- Peptidoglycan types of bacterial cell walls and their taxonomic implications.1972
- Structural Investigations on Cell Walls of Nocardia sp.European Journal of Biochemistry, 1972
- Occurrence of N-glycolylmuramic acid in bacterial cell walls: A preliminary surveyBiochimica et Biophysica Acta (BBA) - General Subjects, 1970
- Structure des parois cellulaires des nocardia. I‐isolement et composition des parois de Nocardia kirovaniFEBS Letters, 1970
- Chemical structure of the cell wall of Mycobacterium smegmatis. I — Isolation and partial characterization of the peptidoglycanBiochemical and Biophysical Research Communications, 1969
- Technique for the preparation of Streptomyces peptidases and N-acetylmuramyl-L-alanine amidase active on bacterial wall peptidoglycansBiochemistry, 1969