This study evaluated the physiological response of rainbow trout to hooking stress after being played under standardized conditions (0-5 min) and estimated the time needed for recovery (to 72 h). Plasma osmolality and Cl- measurements were used to evaluate osmoregulatory disturbances and gill ion-exchange function, and plasma glucose was used as an index of the generalized nonspecific physiological stress response. Hooking stress caused more severe blood chemistry differences in hatchery fish than in wild trout. Hooking stress also imposed a greater stress on larger than on smaller hatchery rainbow trout. Higher water temperatures aggravated the delayed hyperglycemia and hyperchloremia in both hatchery and wild trout but only about 3 days were needed for recovery at 4, 10 or 20.degree. C.