SIMULTANEOUS ASSAY BY 6 METHODS OF EFFECT ON HEMATOPOIETIC PRECURSOR CELLS OF ADRIAMYCIN, METHYL CCNU, CO-60 GAMMA-RAYS, VINBLASTINE, AND CYTOSINE-ARABINOSIDE

  • 1 January 1978
    • journal article
    • research article
    • Vol. 6  (1) , 2-8
Abstract
The spleen colony assay, 3 agar colony assays and 2 repopulating assays were employed to measure the effect of single doses of adriamycin, methyl CCNU [1-(2-chloroethyl)-3,4(4-methylcyclohexyl)-1-nitrosourea], 60Co .gamma.-rays, vinblastine and repeated injection of cytosine arabinoside on early hemopoietic precursors [in mice]. There was no consistent correlation between the effect of these agents on the 6 assays, and the differences in proliferation rate of the cells contributing to the various assays was not demonstrated by the phase specific agents vinblastine and cytosine arabinoside. The relatively high survival of granulocyte repopulating ability after adriamycin and methyl CCNU indicated that the capacity for differentiation and maturation can be influenced by cytotoxic agents so that colony assays while estimating the number of progenitor cells surviving do not necessarily provide a reliable guide to the capacity of the cells for restoring hemopoiesis. Comparison of colony formation by normal bone marrow cells with the 3 agar colony assays showed that the agar diffusion chamber method gave 3 times as many colonies and a much smaller variation between experiments compared to the in vitro agar assay with endotoxin mouse serum as colony stimulating factor. The thin layer feeder cell method gave an intermediate number of colonies.

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