Ouabain-sensitive insulin stimulation of ion and glucose transport in rat ventricular slices.

Publisher Website
Google Scholar
Abstract
Whether ouabain could inhibit insulin-stimulated ion and/or glucose transport in rat ventricular slices was determined. In the slices which were obtained by tearing with forceps from the inside surface of the ventricle, insulin (0.1-100 ng/ml, 20 min treatment) stimulated ATP hydrolysis (nmol Pi/mg tissue per 20 min) and 86Rb uptake (nmol Rb/mg tissue per 8 min) in a concentration-dependent fashion by up to about 25%, compared with control experiment. About 10-fold higher concentrations of insulin stimulated glucose-[14C] uptake by the slices (nmol glucose/mg tissue per 30 min). Both the stimulant effects of insulin on ion and glucose transport were inhibited by ouabain (0.1-1 mM, 20 min treatment) in a similar concentration-response relationship. It never appears that 2 or more independent transmembrane signals would be generated simultaneously in response to the insulin-receptor interaction to operate ion and glucose transport systems in rat cardiac muscle. Rat ventricular slices provided a sensitive tool for the evaluation of insulin effects on ion transport systems in the muscle.

This publication has 2 references indexed in Scilit: