A sequential screening test based on the running component of audiogenic seizures in mice, including reference compound PD50 values

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Abstract
1 . The running component of audiogenic seizures in mice has been used as the basis of a sequential screening test for the detection of a variety of centrally acting drugs. 2 . For acceptance by the test, an active compound must completely suppress the running component in a total of sixteen mice at a dose of 1/5 LD50 intraperitoneally. 3 . Considerable economies in the numbers of animals required for screening have been achieved, the mean number of mice required to reject an inactive compound being 2ṁ0. 4 . The running component is highly sensitive to anticonvulsants and general central depressants, and insensitive to phenothiazine tranquillizers and morphine. Reserpine caused an increase in the severity of the running component. 5 . The statistical model used in this test is of general application to screening test situations which use quantal data.