Replication of Simian Virus 40 Chromatin in vitvo Depends on the Amount of DNA Polymerase alpha. Associated with Replicating Chromatin

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Abstract
Replication of SV40 chromatin in vitro is inhibited by chloride, but stimulated by acetate anions even at physiological concentrations of 100-200 mM. In a similar fashion DNA polymerase .alpha. is affected with respect to the activity with activated DNA as primer template. At concentrations of 100-200 mM acetate, DNA polymerase .alpha. remains associated with replicating chromatin; association is strongly reduced when chloride anions are used at the corresponding concentrations. Thus, the salt behavior of DNA polymerase .alpha. explains the salt sensitivity of the replication system. The importance of this enzyme for DNA replication was confirmed.