An Electrophoretically Cryptic Alcohol Dehydrogenase Variant in Drosophila Melanogaster. 11. Post-Electrophoresis Heat-Treatment Screening of Natural Populations

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Abstract
The 2 common genetic variants of alcohol dehydrogenase (ADH) in D. melanogaster, ADH-F and ADH-S, differ in substrate specificity and electrophoretic mobility. A 3rd inherited variant, ADH-FCh.D., has a substrate specificity like ADH-S, an electrophoretic mobility like ADH-F but much greater thermostability than either of the others. ADH-FCh.D. can be identified after post-electrophoresis heat treatment (15 s at 43.degree. C) on cellulose acetate sheets. The AdhFCh.D. allele was found in 19 of 34 natural populations in Australasia, but its frequency in these populations does not exceed 0.06. The partial correlation between AdhFCh.D. frequency and a maximum temperature variable is significant and positive among the Australasian populations, although different climatic associations are found for a thermostable form of ADH-F in North America.