Down regulation of the mercury resistance operon by the most promoter-distal gene merD

1 December 1989

journal article
research article
Published by Springer Nature in Molecular Genetics and Genomics

Vol. 220 (1) , 69-72
https://doi.org/10.1007/bf00260858

Abstract

The effect of the merD gene on the expression of the mer operon was determined from the rates of accumulation of merA-lacZ fusion protein in the presence and absence of an active merD gene in trans. In the presence of the merD gene, β-galactosidase activity was 2- to 4-fold lower. The merD gene was cloned in a T7 promoter expression vector and the MerD protein product was visualized by autoradiography.

This publication has 18 references indexed in Scilit:

Plasmid-Mediated Heavy Metal Resistances
Annual Review of Microbiology, 1988
Cloning and DNA sequence of the mercuric- and organomercurial-resistance determinants of plasmid pDU1358.
Proceedings of the National Academy of Sciences, 1987
[10] DNA sequencing: A new strategy to create ordered deletions, modified M13 vector, and improved reaction conditions for sequencing by dideoxy chain termination method
Published by Elsevier ,1987
[13] Practical aspects of preparing phage and plasmid DNA: Growth, maintenance, and storage of bacteria and bacteriophage
Published by Elsevier ,1987
Organization, Expression, and Evolution of Genes for Mercury Resistance
Annual Review of Microbiology, 1986
Polypeptides specified by the mercuric resistance (mer) operon of plasmid R100
Gene, 1986
The nucleotide sequence of the mercuric resistance operons of plasmid R100 and transposon Tn501: further evidence for mer genes which enhance the activity of the mercuric ion detoxification system
Molecular Genetics and Genomics, 1986
A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.
Proceedings of the National Academy of Sciences, 1985
Cloning vectors derived from the Pseudomonas plasmid pVS1
Gene, 1985
Mercuric ion-resistance operons of plasmid R100 and transposon Tn501: the beginning of the operon including the regulatory region and the first two structural genes.
Proceedings of the National Academy of Sciences, 1984