APPLICATION OF A SIMPLE GAS-CHROMATOGRAPHIC TECHNIQUE FOR MEASURING BREATH HYDROGEN

  • 1 January 1977
    • journal article
    • research article
    • Vol. 90  (5) , 856-862
Abstract
A simple gas chromatographic technique for analyzing H2 in expired air with a compact, relatively inexpensive gas chromatograph specifically adapted for H2 analysis is described. Modifications in the basic chromatograph included increasing the capacity of the sample loop, lengthening the column and using argon as the carrier gas to increase the sensitivity of the system. Methods for collecting and storing respiratory gas samples and for quantifying the results are described in humans for clinical studies of carbohydrate malabsorption. Small errors in collection and quantitation are introduced by the method, but they are minor in relation to the changes in H2 concentration which are seen with significant malabsorption. This simplification of chromatograph technology could increase the general availability of this convenient, noninvasive and well-tolerated test of intestinal absorption of carbohydrates such as lactose.