Solubilization and purification of aldehyde-generating fatty acyl-CoA reductase from green algaBotryococcus braunii
- 14 August 1995
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 370 (1-2) , 15-18
- https://doi.org/10.1016/0014-5793(95)00781-4
Abstract
Membrane-bound fatty acyl-CoA reductase from the green alga Botryococcus braunii has been solubilized from the microsomal preparation by 0.1% octyl β-glucoside and purified to near homogeneity by Blue A agarose and palmitoyl-CoA agarose affinity column chromatography. The molecular mass of the enzyme was estimated by SDS-PAGE to be 35 kDa. The enzyme generates fatty aldehyde by reduction of fatty acyl-CoA with NADH as the reductant. The N-terminal amino acid sequence of this protein that represents the first eucaryotic aldehyde-generating reductase to be purified shows high homology with the N-terminus of fatty acid reductase from bacteria.Keywords
This publication has 17 references indexed in Scilit:
- Solubilization, Purification and Characterization of Fatty Acyl-CoA Reductase from Duck Uropygial GlandBiochemical and Biophysical Research Communications, 1995
- Nucleotide Sequence of the luxC Gene Encoding Fatty Acid Reductase of the lux Operon from Photobacterium leiognathiBiochemical and Biophysical Research Communications, 1993
- Enhancement of diapausing flesh fly puparia with additional hydrocarbons and evidence for alkane biosynthesis by a decarbonylation mechanismInsect Biochemistry and Molecular Biology, 1992
- Alkane biosynthesis by decarbonylation of aldehyde catalyzed by a microsomal preparation from Botryococcus brauniiArchives of Biochemistry and Biophysics, 1991
- Lipid composition of a green alga, Botryococcus braunii.Agricultural and Biological Chemistry, 1987
- Bacterial bioluminescence: Isolation and genetic analysis of functions from Vibrio fischeriCell, 1983
- Properties of microsomal acyl coenzyme A reductase in mouse preputial glandsArchives of Biochemistry and Biophysics, 1982
- Partial purification and properties of acyl-CoA reductase from Clostridium butyricumArchives of Biochemistry and Biophysics, 1978
- Enzymatic synthesis of fatty alcohols in Brassica oleraceaArchives of Biochemistry and Biophysics, 1971
- Reduction of fatty acids to alcohols by cell-free preparations of Euglena gracilisBiochemistry, 1970