An improved technique of preparing bone-marrow specimens for cytogenetic analysis
- 1 June 1978
- journal article
- research article
- Published by Springer Nature in In Vitro Cellular & Developmental Biology - Plant
- Vol. 14 (6) , 510-515
- https://doi.org/10.1007/bf02616092
Abstract
Sixty-six bone-marrow specimens, derived from patients with hematological and non-hematological disorders, were processed for cytogenetic analysis. Modifications of various parameters of the standard (direct) culture procedure were investigated and the quality of the preparations determined. As a result of these experiments, an improved culture technique was developed that yielded significantly better quality chromosome preparations. This method is based on a short incubation (25-min) of the bone-marrow specimen, immediately following aspiration, in a solution containing both hypotonic KCl and colcemid and completely omits the use of tissue culture medium.This publication has 12 references indexed in Scilit:
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