Activator recruitment by the general transcription machinery: X-ray structural analysis of the Oct-1 POU domain/human U1 octamer/SNAP190 peptide ternary complex
Open Access
- 1 November 2002
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 16 (21) , 2772-2777
- https://doi.org/10.1101/gad.1021002
Abstract
Transcriptional activation of the human U1 snRNA genes is dependent on a noncanonical octamer element contained within an upstream enhancer. The U1 octamer only weakly recruits the Oct-1 POU domain, although recruitment is stimulated by a peptide containing the Oct-1-binding domain of SNAP190. Structural analysis of the Oct-1 POU domain/U1 octamer/SNAP190 peptide complex revealed that SNAP190 makes extensive protein contacts with the Oct-1 POU-specific domain and with the DNA phosphate backbone within the enhancer. Although SNAP190 and OCA-B both interact with the Oct-1 POU domain through the same Oct-1 interface, a single nucleotide within the U1 octamer ablates OCA-B recruitment without compromising activator recruitment by SNAP190.Supplemental material is available athttp://www.genesdev.org.Keywords
This publication has 27 references indexed in Scilit:
- Small Nuclear RNA Genes: a Model System to Study Fundamental Mechanisms of TranscriptionJournal of Biological Chemistry, 2001
- Structure of Pit-1 POU domain bound to DNA as a dimer: unexpected arrangement and flexibility.Genes & Development, 1997
- Basal promoter elements as a selective determinant of transcriptional activator functionNature, 1995
- OBF-1, a novel B cell-specific coactivator that stimulates immunoglobulin promoter activity through association with octamer-binding proteinsCell, 1995
- AMoRe: an automated package for molecular replacementActa Crystallographica Section A Foundations of Crystallography, 1994
- Targeting TBP to a non-TATA box cis-regulatory element: a TBP-containing complex activates transcription from snRNA promoters through the PSE.Genes & Development, 1993
- SETOR: Hardware-lighted three-dimensional solid model representations of macromoleculesJournal of Molecular Graphics, 1993
- A novel B cell-derived coactivator potentiates the activation of immunoglobulin promoters by octamer-binding transcription factorsCell, 1992
- Promoter-selective activation domains in Oct-1 and Oct-2 direct differential activation of an snRNA and mRNA promoterCell, 1992
- RIBBONS 2.0Journal of Applied Crystallography, 1991