Perforated postsynaptic densities: probable intermediates in synapse turnover.

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Abstract
The molecular layer of the dentate gyrus of normal rats shows a large incidence of perforated postsynaptic densities (PSD). The perforations or discontinuities occur almost exclusively in PSD located in spines showing a U- or W-shaped junctional profile (complex PSD). Perforated PSD account for 16-25% of the total complex PSD profiles in young adult rats and 12-29% of those in aged animals. The frequency of perforations in the inner molecular layer of the dentate gyrus undergoes significant changes during a cycle of nondegenerative synapse turnover induced by ipsilateral ablation of the entorhinal cortex. During the first 2 days postlesion nonperforated PSD (simle PSD) decrease sharply, whereas perforated PSD change little. At later times (4-10 days), there is a significant increase in the nuber of perforated PSD that balances the number of simple PSD lost. Beyond 10 days postlesion, the proportion of both types of PSD is restored slowly to normal, i.e., nonperforated PSD increase in number and perforated PSD decrease, returning to the values in unoperated animals by 120 days postlesion. This inverse relationship between small nonperforated PSD and large perforated PSD suggests a precursor-product relationship between them. Perforated PSD are intermediates in an ongoing cycle of synapse turnover that is a part of the normal maintenance and adaptation of the nervous system.