Improvement of Chemical Analysis of Antibiotics. II. Evaluation of Prepacked C18 Cartridge for Extraction of Tetracyclines

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Abstract
The common tetracyclines (TC) in fish tissues which are used in agriculture, tetracycline, oxytetracycline and chlortetracycline, were analyzed using an ODS-cartridge extraction device, and high performance thin layer chromatography (HPTLC) followed by densitometry. Fish tissue was blended in 0.1 M disodium ethylenediaminetetraacetate (di-NaEDTA)-McIlvaine buffer (pH 4.0) and centrifuged. The supernant was adsorbed on the pretreated ODS-cartridge with 0.2 M di-Na EDTA aqueous solution, the cartridge was washed with water, and TC were eluted with ethanol. The concentrated residue is spotted on a silica gel HPTLC plate predeveloped with saturated di-Na EDTA aqueous solution. The plate was then developed with chloroform-methanol-5% di-Na EDTA aqueous solution (65 : 20 : 5, lower layer). The amounts of TC present on the developed plate were determined by UV absorption densitometry. Recoveries of TC from fish samples fortified at level of 1 ppm are 60.2-78.6%.