Expression of ??-synuclein in a human glioma cell line and its up-regulation by interleukin-1??
- 1 July 2001
- journal article
- Published by Wolters Kluwer Health in NeuroReport
- Vol. 12 (9) , 1909-1912
- https://doi.org/10.1097/00001756-200107030-00028
Abstract
Although α-synuclein is expressed primarily in neurons, it is a major component of oligodendroglial and astrocytic inclusions in several neurodegenerative diseases. Recent study has further demonstrated that α-synuclein is expressed in cultured rat oligodendrocytes. We determined whether α-synuclein might be expressed in astrocytic cells. α-Synuclein mRNA and protein were detected in U251 human astrocytic glioma cells and normal human astrocytes, and the levels were increased in the former, but not in the latter, by stimulation with interleukin-1β in a time- and concentration-dependent manner. Serum deprivation also led to an increase of α-synuclein mRNA and protein in U251 cells. Immunofluorescent staining confirmed the cell-associated α-synuclein. These findings suggest that human astrocytes can produce α-synuclein in culture and that certain inflammatory cytokines and cell stress increase α-synuclein expression.Keywords
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