Role of benzylsuccinate in the induction of thetutE tutFDGHgene complex ofT. aromaticastrain T1
Open Access
- 1 February 2004
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 231 (2) , 261-266
- https://doi.org/10.1016/s0378-1097(04)00005-9
Abstract
Expression of the tutE tutFDGH gene cluster of Thauera aromatica strain T1 was examined by Northern and Western analysis in a wild-type strain and chromosomally deleted strains with or without in-frame deletion plasmids. While expression was observed when the wild-type strain was induced with toluene, various chromosomally deleted strains exhibited little or no expression of the tut genes. In contrast, both wild-type and chromosomally deleted strains expressed the tut genes when induced with benzylsuccinate. We conclude that benzylsuccinate is required for the full induction of the tutE tutFDGH gene cluster of T. aromatica strain T1.Keywords
This publication has 33 references indexed in Scilit:
- Construction and characterization of insertion/deletion mutations of thetutF,tutD, andtutGgenes ofThauera aromaticastrain T1FEMS Microbiology Letters, 2002
- Operon structure and expression of the genes for benzylsuccinate synthase in Thauera aromatica strain K172Archiv für Mikrobiologie, 2001
- Benzylsuccinate Synthase of Azoarcus sp. Strain T: Cloning, Sequencing, Transcriptional Organization, and Its Role in Anaerobic Toluene and m -Xylene MineralizationJournal of Bacteriology, 2001
- A two-component system involved in regulation of anaerobic toluene metabolism in Thauera aromaticaFEMS Microbiology Letters, 1998
- Identification of denitrifier strain T1 as Thauera aromatica and proposal for emendation of the genus Thauera definitionInternational Journal of Systematic and Evolutionary Microbiology, 1998
- Evidence That Anaerobic Oxidation of Toluene in the Denitrifying Bacterium Thauera aromatica is Initiated by Formation of Benzylsuccinate from Toluene and FumarateEuropean Journal of Biochemistry, 1996
- Biotransformations of selected alkylbenzenes and halogenated aliphatic hydrocarbons in methanogenic aquifer material: a microcosm studyEnvironmental Science & Technology, 1986
- Measurement of protein using bicinchoninic acidAnalytical Biochemistry, 1985
- lac repressor-operator interactionJournal of Molecular Biology, 1972
- Specificity of the induction of the enzymes of the lac operon in Escherichia coliJournal of Molecular Biology, 1964