High quality draft sequences for prokaryotic genomes using a mix of new sequencing technologies
Open Access
- 16 December 2008
- journal article
- research article
- Published by Springer Nature in BMC Genomics
- Vol. 9 (1) , 603
- https://doi.org/10.1186/1471-2164-9-603
Abstract
Massively parallel DNA sequencing instruments are enabling the decoding of whole genomes at significantly lower cost and higher throughput than classical Sanger technology. Each of these technologies have been estimated to yield assemblies with more problematic features than the standard method. These problems are of a different nature depending on the techniques used. So, an appropriate mix of technologies may help resolve most difficulties, and eventually provide assemblies of high quality without requiring any Sanger-based input.Keywords
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