A Genetic Variant of Factor IX with Decreased Capacity for Ca2+ Binding

Abstract
Summary. A genetic variant of factor IX is described that behaves identically to PIVKA IX (the precursor factor IX molecule induced by the absence of vitamin K or presence of vitamin K antagonists, acarboxy factor IX). It shows an increased electrophoretic mobility in the presence of Ca2+, a low affinity for adsorption to A1(OH)3 and a very low specific coagulant activity. This variant of factor IX has been demonstrated in the plasma of a patient with severe haemophilia B and in the plasmas of a number of possible carriers from the probands’ pedigree.