Observation on the Mechanism of Action of Dicoumarol

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Abstract
The clotting time was studied when dicoumarol plasma was mixed with whole normal plasma, Seitz-filtered plasma, defibrinated plasma, and serum. Dilutions were made with 0.9% NaCl; 0.1 ml. of dicoumarol plasma and 0.1 ml. of diluted normal plasma were added to 0.2 ml. of thromboplastin followed by 0.2 ml. of 0.025 [image] CaCl2. The clotting time was measured at 37[degree]C and the corresponding prothrombin percentage read from a dilution curve. This value was compared with the arithmetic avg. of the prothrombin concns. of the dicoumarol plasma and the normal plasma, measured separately. When normal plasma was added to dicoumarol plasma, the prothrombin content of the mixture was increased more than could be explained by the simple mixing of prothrombin solns. of different concns. The observation could be explained by the presence of a prothrombin accelerator in normal plasma which is deficient in dicoumarol plasma. The accelerator is removed by Seitz filtration. The accelerator disappears when normal plasma is treated with thrombin. It is present, however, in serum.

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