Effects of Vitamin E Deficiency on the Relative Incorporation of 14C-Arachidonate into Platelet Lipids of Rabbits

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Abstract
The potential influence of vitamin E in vivo on the transformation of 14C-arachidonate (14C-ArA) in washed platelets from vitamin E-depleted, pair-fed control and ad libitum-fed control rabbits was investigated. We considered rabbits vitamin E-depleted when their plasma total tocopherol levels were P < 0.01) plasma and platelet tocopherol levels and significantly higher (P < 0.01) urinary creatine:creatinine ratios than those of controls. Thrombin treatment of platelet suspensions that were previously incubated with 14C-ArA resulted in significantly greater (P < 0.05) losses of radiolabel from phospholipids (PL) and significantly greater (P < 0.05) 14C incorporation into prostaglandin E2 (PGE2) fractions from deficient animals than those of either control group. The significantly greater (P < 0.05) losses in lipid phosphorus content of phosphatidylcholine (PC) fractions corroborated the significantly diminished (P < 0.05) radiolabel detected in the PC fraction of deficient animals with thrombin activation. Such findings suggest an enhancement of thrombin-induced phospholipase A2 (PLPA) activity in platelets of vitamin E-deficient rabbits compared to that of controls.