Independence of beta-adrenergic stimulation of renin release on renin synthesis
- 1 November 1982
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Renal Physiology
- Vol. 243 (5) , F434-F439
- https://doi.org/10.1152/ajprenal.1982.243.5.f434
Abstract
Rat renal cortical slices incubated in vitro released 7.0 .+-. 0.5% (mean .+-. SE, n = 30) of their total renin content into the incubation medium in 1 h. Isoproterenol (10-6 M), a .beta.-adrenergic agonist, caused a 75 .+-. 17% (n = 7) increase in renin release from the cortical slices. Treatment of the cortical slices with either cycloheximide or puromycin caused a significant 96.2 .+-. 0.34 (n = 8) or 98.5 .+-. 0.3% (n = 5), respectively, inhibition of protein synthesis. After protein synthesis and presumably renin synthesis were blocked with cycloheximide or puromycin, isoproterenol was still able to significantly increase renin release from cortical slices despite almost total blockade of protein synthesis. A storage pool of renin may exist, since only 7.0 .+-. 0.5% of the cortical slice renin content is released each hour, and isoproterenol stimulation of renin release is not acutely dependent on renin synthesis. .beta.-Adrenergic stimulation of renin release may be elicited from a storage pool of previously synthesized renin.This publication has 0 references indexed in Scilit: