Characterisation by molecular hybridization of RNA fragments isolated from ancient (1400 B.C.) seeds
- 1 December 1985
- journal article
- research article
- Published by Springer Nature in Theoretical and Applied Genetics
- Vol. 71 (2) , 330-333
- https://doi.org/10.1007/bf00252076
Abstract
The analysis of cress seeds from Thebes dated approximately 1400 years B.C. showed that fragments of RNA up to 10 bases in length were still present in the ancient seeds. After having been made radioactive at the 5′OH terminus, the RNA fragments were used as probes in a spot hybridization experiment. They were shown to hybridize to cress DNA and, to a lesser extent, to that of phylogenetically distant species. When fixed onto nitrocellulose and probed with different cloned genes, the RNA fragments were shown to originate from breakage of the 25 and 18s cytoplasmic rRNA.Keywords
This publication has 9 references indexed in Scilit:
- DNA sequences from the quagga, an extinct member of the horse familyNature, 1984
- The application of spot hybridization to the detection of DNA and RNA viruses in plant tissuesJournal of Virological Methods, 1983
- A COMPARISON OF THE PROTEIN AND AMINO ACID COMPOSITION OF OLD AND RECENT BARLEY GRAINNew Phytologist, 1982
- Characterisation of the genes for ribosomal RNA in flaxNucleic Acids Research, 1981
- Nucleic acid spot hybridization: rapid quantitative screening of lymphoid cell lines for Epstein-Barr viral DNA.Proceedings of the National Academy of Sciences, 1980
- THE EXTRACTION, COMPOSITION AND INTRA-CELLULAR DISTRIBUTION OF PROTEIN IN EARLY MAIZE GRAINS FROM AN ARCHAEOLOGICAL SITE IN N.E. ARIZONANew Phytologist, 1977
- Similarities among ribosomal RNA's of angiospermae and gymnospermaeJournal of Molecular Evolution, 1976
- Nucleotide sequence of the rightward operator of phage lambda.Proceedings of the National Academy of Sciences, 1975
- Analysis of Barley from King Tuthankhamen's TombNature, 1944