Isolation and reconstitution of the high‐affinity choline carrier
Open Access
- 13 March 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 245 (1-2) , 235-237
- https://doi.org/10.1016/0014-5793(89)80228-5
Abstract
Monoclonal antibodies, which block the high‐affinity uptake of choline in synaptosomal ghosts, have been used to purify a membrane polypeptide (80 kDa) from insect synaptosomal membranes. This isolated protein was found to catalyse the sodium‐dependent, hemicholinium‐sensitive accumulation of choline after reconstitution into liposomes, thus, apparently represents the high‐affinity choline transporter.Keywords
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