Insulin-Like Growth Factor-I Action on Hypothyroid Rat Pituitary Cells: Suppression of Triiodothyronine- Induced Growth Hormone Secretion and Messenger Ribonucleic Acid Levels*

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Abstract
T3 stimulates in vitro GH secretion, whereas somatomedins have been shown to suppress GH. We, therefore, tested the effects of a recombinant human insulin-like growth factor analog (IGF-I; Thr 59) on T3-induced GH secretion. Pituitary monolayer cultures were derived from 4- to 7-week surgically thyroidectomized male rats and grown in hypothyroid medium stripped of T3 and T4. Hypothyroid pituitary cell monolayers secreted low levels of GH (200 ng/ml-72 h). GH secretion was stimulated (185% of T3-free controls) by the addition of T3 (0.1 nM) during 48 h of incubation. IGF-I (up to 13 nM) did not alter basal GH secretion, but inhibited the induction of GH by T3. IGF-I (0.65 nM) suppressed the 3.5-fold induction of GH by 0.25 nM T3 by 35% (p 3-induced GH by IGF-I occurred after 24 h. By 72 h, IGF-I (3.25 nM) suppressed GH stimulation by up to 1 nM T3 [213 ± 12 (±SE) vs. 88 ± 8 ng GH/104 cells]. Insulin, epidermal growth factor, and fibroblast growth factor did not alter the T3- induced GH secretion. GH mRNA was measured by agarose gel blot hybridization analysis and nitrocellulose dot blot hybridization of cytoplasmic RNA with rat GH [32P]cDNA. T3 (0.25 nM) stimulated the hybridization of immobilized pituitary cell RNA extracts, indicating an almost 3-fold increase in rat (r) GH mRNA levels; IGF-I (3.25 nM) suppressed the T3-induced rGH mRNA content of pituitary cells from thyroidectomized rats by 50% during 72 h of incubation. Maximal suppression of rGH mRNA induced by 0.25 nM T3 was achieved with 6.5 nM IGF-I (35% of T3-treated cells). These results show that IGF-I does not alter low basal GH secretion in pituitary cells from hypothyroid rats. IGF-I does, however, block the T3-induction of GH secretion and suppresses GH mRNA levels stimulated by T3. As T3 is known to directly stimulate GH gene transcription, IGF-I may inhibit GH gene transcription. Alternatively, IGF-I may suppress T3 action on GH at a posttranscriptional site. (Endocrinology118: 1483–1490, 1986)

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