A NOVEL GROUP OF GLYCOLIPIDS IN TISSUES OF APLYSIA: TWO-DIMENSIONAL THIN-LAYER CHROMATOGRAPHIC ANALYSIS

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Abstract
Water-soluble lipids of the skin, ganglia and nerve fibers of the sea mollusc, a sea hare, Aplysia kurodai were analyzed by two-dimensional thin-layer chromatography. With anthrone reagent, 20 to 30 spots of glycolipids were detected in chromatograms of these tissues. Most of the glycolipids also gave a positive reaction with Dittmer-Lester''s reagent for phosphorus and with the fluorescamine reagent for free amino groups. These chromatographic findings, data on chemical and amino acid analyses of water-soluble lipid fractions from the three tissues, and the results of structural studies on the major skin glycolipid, SGL-II, indicated that most, if not all, of the water-soluble lipids of the tissues of Aplysia are phosphonoglycosphingolipids containing 2-aminoethylphosphonic acid. Some phosphonoglycosphingolipids seemed to be specific to, or specifically concentrated in certain tissues, and each tissue showed a characteristic pattern of phosphonoglycosphingolipids on two-dimensional thin-layer chromatography.