A QUANTITATIVE DETERMINATION OF THE CHANGES IN HEMOGLOBIN CONCENTRATION, VOLUME OF RED CELLS, AND BASOPHILIA IN THE BLOOD OF RABBIT FETUSES AT VARIOUS STAGES DURING THE LAST THIRD OF PREGNANCY

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Abstract
The general plan in the present investigation was to determine the hemoglobin content of the blood by Kennedy''s method, using a Du Boscq colorimeter. This colorimetric estimate was checked by histological examination of the red cells in smears prepared by Jenner''s modified Romanowsky stain, and the proportion of basophilic red cells was counted. Further verification of the hemoglobin concentration was obtained by hema-tocrit determinations giving the volume of red cells per unit volume of blood. In all cases the blood of the mother was studied, as well as of the fetuses. Observations were made on 87 fetuses of 19 rabbits during the last 3rd of gestation. It was found that the hemoglobin concentration increased from an average of about 8 gm. per 100 cc. of blood at 22 days to about 12 gm. per 100 cc. of blood at term, or roughly an increase of 50%. The volume of red corpuscles increased from about 28 volumes % at 22 days to about 42 volumes % at term, or roughly an increase of 50%. The percentage of basophilic red cells in histological preparations decreased from about 28% at 22 days to about 4% at term.

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