Cell-free synthesis of polyoma virus capsid proteins VP1 and VP2

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Abstract
Polyadenylated RNA isolated from the cytoplasm of mouse [fibroblast] 3T6 cells 28 h after infection with polyoma virus was isolated and translated in vitro. Polyoma capsid proteins VP1 and VP2 were identified in the cell-free product by polyacrylamide gel electrophoresis, specific immunoprecipitation and tryptic peptide fingerprinting. Polyoma mRNA species, isolated by preparative hybridization to purified viral DNA immobilized on cellulose nitrate filters, coded for VP1 and VP2. These experiments establish conditions for the isolation of late polyoma mRNA and the cell-free synthesis of polyoma capsid proteins and indicate that the active mRNA species are at least partially virus coded.