The Aseptic Culture of Isolated Buds of Pseudotsuga Taxifolia.

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Abstract
A technique is described for sterilizing buds taken from the trees in the middle of the winter rest period and culturing them on an agar nutrient medium. Such isolated buds require available O2 and C and energy source, preferably sucrose, and mineral elements. Buds fail to develop in the absence of light but develop well on a nutrient medium if grown under white fluorescent light. Added auxins at 10-6 [image] had no stimulatory effect on bud or leaf expansion; higher concentrations were inhibitory to bud development and stimulated callus tissue proliferation at the base of the bud. Gibberellic acid, although stimulating axial elongation, always caused death of the bud. Of a number of organic substances tested, only urea had a beneficial effect on bud development. Other organic supplements, including coconut milk, yeast extract, casein hydrolysate and watermelon juice were inhibitory to bud expansion. On the bast medium developed, isolated buds were maintained for over 2 months in vitro during which they developed from tiny naked buds to large, partially-expanded shoot systems bearing mature, fully-expanded basal leaves.