The extraction of pigments from Euglena gracilis

Abstract
Pigments were extracted from cultures of Euglena gracilis by means of an acetone-ethyl acetate-solvent system. This technique permits the measurement of growth response of this alga over a wider range of vitamin B12 concentrations than has hitherto been possible. A direct relationship has been demonstrated between pigment concentration and cell size. The application of the solvent-system technique to vitamin B12-antimetabolite studies is illustrated by an experiment with 6-mercaptopurine.

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