Immuno-Electron Microscopic Localization of the Encephalitogenic Basic Protein in Myelin

Abstract
Immuno-electron microscopic studies of the localization of encephalitogenic basic protein demonstrate its presence in the major dense line of the myelin sheath. Staining could only be achieved where the myelin was partially disrupted due to a failure of the labelled antibody to penetrate the intact myelin sheath. This finding supports the view that the antigenic determinants of the basic protein are occluded in vivo.