Étude ultrastructurale des tubes polliniques angiospermiens : application de quelques techniques cytochimiques

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Abstract
The use of a variety of cytochemical techniques (test of PATAg, staining with phosphotungstic acid, Swift's reaction, digestion with pectinase, extraction with EDTA and dimethyl sulfoxide (DMSO)) affords a better approach to the study of the mechanism of the growth of pollen tubes. Vesicles produced by the Golgi apparatus accumulate in the tip of the pollen tubes. Their membranes are gradually transformed and become cytochemically similar to the plasmalemma with which they fuse, participating in its renewal. Their contents contribute to the tube wall formation. The structure of the tube wall is complex and appears to be formed of a loose matrix of cellulose fibrils impregnated with pectic materials.