Nucleotide sequence of a cluster of early and late genes in a conserved segment of the vaccinia virus genome
Open Access
- 1 January 1985
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 13 (3) , 985-998
- https://doi.org/10.1093/nar/13.3.985
Abstract
The nucleotide sequence of a 7.6 kb vaccinia DNA segment from a genomic region conserved among different orthopox virus has been determined. This segment contains a tight cluster of 12 partly overlapping open reading frames most of which can be correlated with previously identified early and late proteins and mRNAs. Regulatory signals used by vaccinia virus have been studied. Presumptive promoter regions are rich in A,T and carry the consensus sequences TATA and AATAA spaced at 20–24 base pairs. Tandem repeats of a CTATTC consensus sequence are proposed to be involved in the termination of early transcription.Keywords
This publication has 11 references indexed in Scilit:
- [57] Sequencing end-labeled DNA with base-specific chemical cleavagesPublished by Elsevier ,2004
- Fine structure analysis and nucleotide sequence of the vaccinia virus thymidine kinase gene.Proceedings of the National Academy of Sciences, 1983
- Vaccinia virus: a selectable eukaryotic cloning and expression vector.Proceedings of the National Academy of Sciences, 1982
- Construction of poxviruses as cloning vectors: insertion of the thymidine kinase gene from herpes simplex virus into the DNA of infectious vaccinia virus.Proceedings of the National Academy of Sciences, 1982
- Organization and expression of the poxvirus genomeCellular and Molecular Life Sciences, 1982
- Distinctive nucleotide sequences adjacent to multiple initiation and termination sites of an early vaccinia virus geneCell, 1981
- Cell-free translation of early and late mRNAs selected by hybridization to cloned DNA fragments derived from the left 14 million to 72 million daltons of the vaccinia virus genomeVirology, 1981
- Purification and characterization of a DNA-dependent RNA polymerase from vaccinia virions.Journal of Biological Chemistry, 1980
- Conservation and Variation in Orthopoxvirus Genome StructureJournal of General Virology, 1979
- A simple method for DNA restriction site mappingNucleic Acids Research, 1976