Graphic stereo‐reconstruction of serial sections

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Abstract
SUMMARY: Serial histological sections may be graphically reconstructed in the form of paired ‘stereo‐maps’ for observation with a stereoscope. Comfortable stereo‐vision is obtained if the contour lines which produces grades are laterally parallaxed to an appropriate degree. Since the technique is advantageous and its procedures are simple in practice, it will be applied in a number of investigations. The basis of the technique is that the parallax is achieved by staggering one of the half‐maps with a micrometer at the rate of one‐tenth the section interval, which gives rise to perspective in the stereo‐vision. Some fundamental conditions, involved in procedures as well as observation, were investigated in order to consider the nature of the final image. Thus, it has proved to be a satisfactory representation. Other than the primary reconstruction, which is observed at right angles to the sectional plane, a method to convert the viewing direction, or the profile cartography, has been developed, so that we are able to observe sectioned structures practically from any direction.

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