Oxygen Consumption Rate of Tissue Measured by a Micropolarographic Method
Open Access
- 1 November 1966
- journal article
- research article
- Published by Rockefeller University Press in The Journal of general physiology
- Vol. 50 (2) , 317-335
- https://doi.org/10.1085/jgp.50.2.317
Abstract
A new method for measuring the oxygen consumption rate of a sheet of homogeneous tissue is described. The method measures, by a Clark-type oxygen electrode without a membrane, the time for the tissue to consume all its dissolved oxygen. The electrode is applied to one surface of the tissue sheet and the other surface is sealed from the atmosphere by a cover slip. The consumption is calculated from an estimate of the oxygen dissolved in the tissue at the moment it is covered and the time for the oxygen tension at one surface to fall to zero. The data also yield the oxygen diffusion coefficient in the oxygen-consuming tissue.This publication has 6 references indexed in Scilit:
- The diffusion of oxygen in the corneaExperimental Eye Research, 1965
- An ultramicro oxygen electrodeJournal of Applied Physiology, 1964
- Oxygen Uptake from a Reservoir of Limited Volume by the Human Cornea in vivoScience, 1963
- The structure and transparency of the corneaThe Journal of Physiology, 1957
- Utilization of oxygen by the component layers of the living corneaThe Journal of Physiology, 1952
- The Steady State of Corneal Hydration*American Journal of Ophthalmology, 1949